Best suited for research environments, applications include fluorometric protein, enzyme, and kinase studies. Fluoroskan ascent fl is a microplate fluorometer and luminometer with excellent optical performance for a variety of research applications, offering versatile plate formats, fast reading speed, up to three dispensers and top bottom reading of plates. Perform a variety of research applications with the compact, robust thermo scientific. Fluoroskan ascent microplate fluorometer luminoskan ascent microplate luminometer fluoroskan ascent fl combined microplate. View pdf catalogues and other online documentation prices are pretax. Institute for molecular medicine, university medical center of the johannes gutenberg, university of mainz, 551 mainz, germany. The fluoroskan ascent, a dedicated microplate fluorometer, offers high sensitivity for a wide variety of fluorometric applications.
This microplate fluorometer is ideal for life science research applications such as fluorometric protein and enzyme studie. The fluoroskan ascent fl is a combined highquality microplate fluorometer and luminometer intended for laboratory research use by professional personnel. Thermo scientific accessories for fluoroskan ascent fl microplate fluorometer and luminometer perform a range of fluorometric and luminometric research applications with thermo scientific fluoroskan ascent fl, offering fast read speed and topbottom plate reading. After a flash of onoff welcome screen, similar to the one shown in figure 1, the screen automatically. Each well was scanned by measuring the intensity of each of 25 squares of 1mm2 area arranged in a 5. The electrons may be present in bonding molecular orbital. May 25, 2019 here my blog where i share pdf files with my readers.
Upload files that contain software or other material protected by intellectual property laws or by rights of privacy of publicity unless you own or control the rights thereto or have received all necessary consents. Molecule contains electrons, electrons and non bonding n electron. Decreased transcriptioncoupled nucleotide excision repair. Tcdd was added to lmat cells that had been loaded with 5. Featuring excellent optical performance, the fluoroskan microplate fluorometer is ideal for life science research applications such as fluorometric protein and enzyme studies, molecular interaction studies, nucleic acid quantification, as well as reporter gene, fluorometric kinase, immunological, and cellbased assays. Fluoroskan ascent fluorescence cf cell reading fluorescence. Measuring fluorescein using the quantus fluorometer. Molecular devices spectramax gemini xs for sale labx.
Impact of dabigatran on a large panel of routine or specific. The emission filter slots 7 and 8 are reserved only. Instrument fluoroskan microplate fluorometer 5200110 fluoroskan fl microplate fluorometer and luminometer 5200220 reagents quantit dsdna assay kit, high sensitivity q33120 microplates. Statins reduce lipopolysaccharideinduced cytokine and. Thermo scientific fluoroskan ascent or fluoroskan ascent fl, this reagent provides rapid and easy determination of dsdna, offering ultimate sensitivity and a wide dynamic range. Thermo fisher scientific the 374 fluoroskan ascent microplate fluorometer features excellent optical performance. Fluorescein can be measured using the quantus fluorometer cat. Compare fluoroskan ascent series microplate readers from thermo fisher scientific on. Used labsystems fluoroskan ascent 2024129 fluorometer. If you dont see the used microplate reader that youre. The fluoroskan ascent fl fluoroskan ascent can carry out both top and bottom reading. The instrument is controlled with the flexible thermo scientific ascent software. Thermo scientific fluoroskan ascent fl from thermo fisher. The difference between them is the way they select the wavelengths of incident light.
Thermo scientific fluoroskan ascent fl the thermo scientific fluoroskan ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, and immuno and cell based assays. Thus, the reader is ideal for applications such as reporter gene and cell adhesion assays, as well as cell proliferation and cytotoxicity assays. The labsystems fluoroskan ascent fl is microplate fluorometer and. Ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as. It features compatibility with 1 to 384 well plate formats and pcr plates, up to three onboard dispensers, an incubator and a shaker. Spectral peaks may shift as a function of temperature, ph, solvent, or coupling chemistry.
The fluoroskan fl microplate fluorometer and luminometer is capable of reading a 96well plate in just 18 seconds, essential for kinetic applications. Highthroughput identification of peptide agonists against. Fluoroskan fl microplate fluorometer and luminometer. In his honor there is a board of recognition by the state of aguascalientes at the base of the column of the exedranext to the fountain from a spring dedicated to this musical poet, in the city of aguascalientes where he grew up and first studied music. Electrophoresis, blotting and immunodetection plate based. Fluorescence intensities were measured on fluoroskan ascent microplate fluorometer. He was married to clementina maurel, next to whom he died in mexico city. Hypoxia induces a prothrombotic state independently of the. The source of the excitation light and the detector of the emission light are both located on the same side of the microplate. An optical system based in fiberless direct illumination gives the instrument a wide range of applications. Pdf fluorescence microplate assay for the detection of oxidative. Fluorometric gfp measurement and luminometric luciferase measurement from the same well occurs quickly due to the versatility of the assay programming. Impact of dabigatran on a large panel of routine or.
Because fluorescein is autofluorescent, no dye chemistries are necessary for quantitation on the quantus fluorometer. For applications such as fluorometric protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, immuno and. Fluoroskan ascent and fluoroskan ascent fl microplate. Thermo scientific fluoroskan ascent the thermo scientific fluoroskan ascent is a microplate fluorometer for life science research applications, such as fluorometric protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, immuno and cell based assays. Antioxidants improve the viability of stored adult retinal pigment. May 06, 2016 one hundred microliters of sample was collected from the abluminal compartment at the end of 30 minutes and measured fluorometrically at 485520 nm excitationemission using fluoroskan ascent fl microplate fluorometer and luminometer vantaa, finland. Fluorescence measurements were carried out in a fluoroskan ascent microplate fluorometer using excitation and emission wavelengths of 355 and 405 nm, respectively.
Oct 30, 2015 samples were analyzed for 50 minutes and fluorescence was recorded every 6 seconds with a fluoroskan ascent microplate fluorometer with. Fluoroskan ascent fl fluorometer with three dispensers, 5210463, ca95043050, each. Cisplatin resistance is multifactorial but can be associated with increased dna repair capacity, mutations in p53 or loss of dna mismatch repair. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. As nouns the difference between fluorimeter and fluorometer is that fluorimeter is a fluorometer while fluorometer is an instrument used to detect and measure fluorescence. Loss of endothelial cell integrity and selective permeability barrier is an early event in the sequence of oxidantmediated injury and may result in atherosclerosis, hypertension and facilitation of transendothelial migration of cancer cells during metastasis.
Thermo scientific fluoroskan ascent fl microplate fluorometer and luminometer is ideal for life science research applications. Microplate instrumentation mib application laboratory, thermo fisher scientific, vantaa, finland introduction the picogreen reagent is an ultra. Up to three reagent dispensers can be fitted onboard, making the reagent addition simple and highly accurate. Upload files that contain software or other material protected by intellectual property laws or by rights. Used thermo scientific fluoroskan ascent microplate. It is measurement of fluorescence intensity at a particular wavelength wit the help of a filter fluorimeter or a spectrofluorimeter. Used thermo fluoroskan ascent 374 fluorometer for sale. The thermo scientific fluoroskan fl is a compact and robust microplate fluorometer and luminometer, offering simplicity and reliability for increased efficiency of workflows.
Our exclusive contracts with our clients yield a wide range of used microplate readers from a number of respected oems, including perkin elmer, tecan, molecular devices, biotek instruments, packard automation, labsystems and more. L with 50 mm tris buffer ph 7 ionic strength adjusted to 100 mm with nacl with a fluorescein filter set ex. Fluoroskan ascent microplate fluorometer pdf thermo scientific fluoroskan ascent microplate fluorometer. Thermo labsystems ascent family covers both fluorometry and luminometry with. Samples were tested in triplicate and calibrated as previously described. The fluoroselect fluorometer can be powered with 4 aa alkaline batteries or a power adapter 5vdc.
Super high resolution for single moleculesequencebased. Thermo scientific luminoskan ascent thermo scientific. Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins. Fluoroskan, fluoroskan fl and luminoskan key features. We already reported that endothelial cell integrity is tightly regulated by the balanced coactivation of p38 and erk pathways. Stimulation of gsh synthesis to prevent oxidative stress. Thermo labsystems 374 fluoroskan ascent microplate fluorometer. The fluoroskan ascent is a highquality microplate fluorometer intended for laboratory. Thermo scientific fluoroskan ascent forums, the thermo scientific fluoroskan ascent is a computercontrolled microplate fluorometer for life science research applications, such as fluorometric protein and other files to download. The fluoroskan ascent fl is equipped with both fluorometric and luminometric measurement technologies, providing a full coverage for fluorometric, glow and flash luminometric applications even in the same run. The 374 fluoroskan ascent microplate fluorometer features excellent optical performance. Automated selfdiagnostics and automatic power save functions offer timesaving convenience. The thermo scientific fluoroskan ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase. Super high resolution for single moleculesequencebased typing of classical hla loci at the 8digit level using next generation sequencers.
Prices are indicative only and may vary by country, with changes to the cost of raw materials and. Fluoroskan ascent microplate fluorometer from thermo. A fluorescencebased protocol for quantifying angiotensin. Labsystems fluoroskan ascent fl equipment information msu. A fluorometer sometimes fluorimeter is a spectrometer designed to measure this phenomenon. Therefore, the assumption that all statin compounds will have the same effect within the central.
A filter fluorometer will use filters while a spectrofluorometer will use grating monochromators. Fluoroskan ascent fl microplate fluorometer and luminometer for both luminometric and fluorometric research applications. Shop online for a wide selection of thermo scientific fluoroskan ascent microplate fluorometer perform a variety of research applications with the. Measurement with the fluoroskan ascent fl and fluoroskan ascent occurs in a narrow angle. Cell phenotype was investigated by both microplate fluorometer and epifluorescence. Fluorometers fluorescence spectrophotometers a fluorescence spectrophotometer or fluorometer, fluorospectrometer, or fluorescence spectrometer measures the fluorescent light emitted from a sample at different wavelengths, after illumination with a xenon flash lamp. The thermo scientific fluoroskan ascent fl is a compact microplate fluorometer and luminometer. Regulation of endothelial permeability and transendothelial. In many molecular biology applications the detection and quantitation of small amounts of dsdna is very important. The position of the whole optic unit is easily changed. Analytical tlc was performed on readytouse plates with silica gel 60 merck.
F luoroskan, fluoroskan fl, and l uminoskan microplate readers. Thermo labsystems multiskan ascent 354 microplate plate reader. Cam fluorescence quantification was performed with a microplate fluorometer fluoroskan ascent, thermo scientific. Standard factoryinstalled filter pair for fluoroskan and fluoroskan fl instruments. The fluoroskan ascent flfluoroskan ascent can carry out both top and bottom reading. Fluoroskan fl microplate fluorometer and luminometer, 100240 v, 5060 hz with two dispensers 5200222 includes pc software and filter pairs. Technical note fluoroskan microplate fluorometer sensitive fluorometric quantification of dna in microplate format materials cat. Thermofisher scientific fluoroskan ascent fl fluorometer. The direct illumination optics of the fluoroskan ascent produces a highly focused light beam. Labsystems multiskan ex manual 2806100, view, unable labsystems to connect to commerce server, parts for multiskan rc ms ex from thermo scientific brand microplate readers manual. Oct 12, 2017 luciferase measurements were carried out as described using a fluoroskan ascent fl microplate fluorometer and luminometer. The onboard dispensers support simultaneous dispensing and reading, enabling the monitoring of fast kinetic. The thermo scientific fluoroskan ascent fl is a microplate fluorometer and luminometer for both luminometric and fluorometric research applications, such as protein and enzyme studies, molecular interactions, nucleic acid quantification, reporter gene, fluorometric kinase, and immuno and cell based assays. The compact fluoroskan ascent cf is simplified and standardized to focus specifically on cellbased requirements.
Luminometric measurement principle fluoroskan ascent fl. One of the most commonly used classes of anticancer drugs presently in clinical practice is the platinumbased drugs, including cisplatin. Recommended filter pairs for various fluorescent dyes. Fluoroskan ascent microplate fluorometer according to the manufacturers protocol. The thermo scientific fluoroskan ascent fl microplate reader is. Fluoroskan fl microplate reader fluorometer and luminometer fluoroskan microplate reader dedicated fluorometer luminoskan microplate reader dedicated luminomter on board dispensing is important for flash chemistry such as atp measurement or calcium flux assays the instrument includes incubation and can be ordered with up to two dispensers.
The fluoroskan ascent figure 4 is an advanced microplate fluorometer with high sensitivity for a wide variety of fluorometric applications. The fluoroskan ascent from labsystems is a microplate fluorometer which. A calibrated automated tool to assess the thrombin. The onboard dispensers support simultaneous dispensing and reading, enabling the monitoring of fast kinetic measurements from the very start of the reaction. Used thermo scientific fluoroskan ascent microplate fluorometer.
This table contains only a partial list of the fluorescent dyes that can be used with the fluoroskan and fluoroskan fl microplate fluorometers. Featuring excellent optical performance, the fluoroskan fl microplate fluorometer and luminometer is ideal for life science fluorometric and luminometric assays. There are two basic types of fluorometers, the filter fluorometer and the spectrofluorometer. Excellence for fluorometric and luminometric measurements. Comes with computer and software, plus some manuals. Dabigatran was tested at nine concentrations ranging from 4. The efficacy of cisplatin therapy is often limited by the emergence of resistant tumours following treatment. The first part of the fluorometer is very similar to the spectrophotometer, and consists of a light source and a filter or monochromator to select out a defined group of excitation wavelengths, which are then directed into a sample. Equipnet is the worlds leading provider of used microplate readers and a number of other used equipment.
The antiinflammatory effects of statins hmgcoa reductase inhibitors within the cardiovascular system are wellestablished. Fluoroskan ascent microplate fluorometer thermo fisher scientific with black, nonbinding 384 well plates greiner at a reaction volume of 60. The only components required are a standard of known concentration and a blank. In fluorescence measurement, this prevents crosstalk and ensures accurate readings. Download pdf copy thermo scientific fluoroskan ascent microplate fluorometer is a compact and robust instrument with excellent optical performance for a variety of research applications.
The thermo scientific fluoroskan ascent fl microplate reader is a. Includes mtx spectrosoft software installation cd, dongle, cover, and cordscables as pictured. Improved method to retain cytosolic reporter protein. Ogr1 probe was synthesized by using an applied biosystems 394 dnarna synthesizer. Fluoroskan ascent microplate fluorometer fluoroskan ascent fl combined microplate fluorometer and luminometer reads 84well plates topbottom reading robotic compatible up to three dispensers incubation and shaking high sensitivity luminoskan ascent microplate luminometer reads 84well plates high. Thermo scientific fluoroskan ascent microplate fluorometer. Supporting information potent and selective inhibitors of. High sensitivity luminometric and fluorometric assays in a. Microplate fluorometers for a variety of uses in the life science field, including dna, rna, and enzyme studies, feature enhanced reading speeds. Crude and purified proteasome activity assays are affected by type.
Fluoroskan ascent series microplate readers from thermo. Microplate fluorometers for a variety of uses in the life science field, including dna, rna, and. Thermo scientific fluoroskan ascent, luminoskan ascent and fluoroskan ascent fl fluoroskan ascent luminoskan ascent fluoroskan ascent fl fluorometry excitation wavelength range 320 700 nm 320 700 nm emission wavelength range 360 800 nm 360 670 nm excitation filters up to eight filters in the excitation filter wheel. Fluoroskan ascent and fluoroskan ascent fl microplate fluorometers, thermo scientific. Highlyvisual ascent software simplifies fluorometric applications flexible plate formats, from 6 to 96 wells and 6 to 384 wells, enable reading in primary wells reads from above or above the plate all models. Over 10 million scientific documents at your fingertips. Fluoroskan ascent microplate fluorometer thermo fisher scienti. The fluoroskan flexible design allows for 196 and 384well plate analysis. It is currently unknown whether statins neurological effects are lipiddependent or due to pleiotropic mechanisms. Labsystems now thermo 374 fluoroskan ascent fluorescence microplate reader itemspecific notes. Before the start of the bril formation reaction, the protein in 10 mm sodium acetate bu. Cell culture all melanoma cell lines in this study were established from metastasis of patients with stage iii or iv melanoma according to ajcc as previously described 17. Portable models are lightweight and compact for use in field studies. The g126v mutation in the mouse prion protein hinders.
1303 141 765 1094 1363 912 1313 907 110 755 280 827 881 1377 142 707 1144 394 589 576 979 991 154 593 612 26 1640 681 1019 1189 626 1188 1365 1570 710 614 399 710 176 110 1431 1261 490 576 194